Srinagar, Feb 10: CSIR–Indian Institute of Integrative Medicine (CSIR-IIIM), Srinagar Br., has inaugurated a three-day hands-on training programme on quantitative real-time PCR (qRT-PCR) to build practical competencies in modern molecular biology techniques among students, researchers, and laboratory professionals.
The Program is designed to equip participants with comprehensive, bench-level proficiency in gene expression analysis from sample to result. In his virtual message, Dr. Zabeer Ahmed, Director, CSIR-IIIM, underscored the central importance of qRT-PCR in molecular biology, noting its role in sensitive transcript quantification, validation of high-throughput data, and support for translational research and diagnostics.
Across three immersive days, the curriculum takes participants through the full experimental arc. From careful isolation of high-quality RNA and contamination control to optimized reverse transcription with considered primer strategies, assay design, and chemistry selection (including SYBR Green and probe-based approaches), rigorous instrument setup and run execution.
The programme emphasizes practical mastery and extended wet lab sessions on RNA isolation, cDNA synthesis, and qRT-PCR, paired with structured troubleshooting to resolve low yields, non-specific amplification, efficiency anomalies, and inter-run variability, as well as good laboratory practices.
The training highlights include hands-on RNA isolation, cDNA synthesis, and qRT-PCR, end-to-end data analysis, and a dedicated troubleshooting clinic, reflecting CSIR IIIM’s commitment to skill development and capacity building in cutting-edge molecular techniques.
The Program is being organized at CSIR-IIIM Srinagar (Br.) by Dr Nasheeman Ashraf, Senior Principal Scientist, and co-organized by Dr. Syed Reyaz ul Hassan, Senior Principal Scientist, and Dr. Phalesteen Sultan, Principal Technical Officer, under the patronage of Er. Abdul Rahim, Chief Scientist and Head, IIIM Srinagar Br.
The programme runs from February 9 to 11, 2026 Under CSIR-Integrated Skill initiatives and around 20 participants from different parts of country are participating in said program. (PIB)
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